Pioneer in phage-based diagnostics

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    • What is Actiphage TB?
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    • Actiphage assay workflow
    • Instructions for use of Actiphage
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Menu
  • Home
  • About
    • About PBD Biotech
    • Leadership
    • Blog
  • Latest
    • Press releases
    • In the news
    • Events
  • Actiphage TB
    • What is Actiphage TB?
    • Unique role in TB testing
    • Supporting test and treat campaigns
    • Clinical studies
  • Lab Support
    • Laboratory Support
    • Solutions for laboratory developed tests
    • Actiphage assay workflow
    • Instructions for use of Actiphage
  • TB Drug Research
    • TB drug research
    • Research Use Only
    • Biomarker for TB prevention
  • Contact
    • Contacting us
    • Partnerships and collaborations

Actiphage assay workflow

Actiphage is a novel bacteriophage-based assay that infects and lyses active mycobacterium tuberculosis (Mtb) to release bacterial DNA for PCR based detection.

Key Actiphage workflow steps

Actiphage blood collection
  • 2ml blood sample (sodium heparin) is required.
  • Not refrigerated or frozen; shipped at ambient = 10-24C.
  • Processed to the start of phage incubation, within 48 hours of collection, to prevent white blood cell degradation.
Actiphage white blood cell isolation
  • Actiphage causes lysis of white blood cells to release the mycobacteria.
DNA is recovered from the mycobacterial DNA
Actiphage qualitative result is determined with qPCR
  • Qualitative result: positive or negative for mycobacterium tuberculosis
  • The PCR reagents targets M. tuberculosis conserved, multicopy gene IS6110 DNA. 

Potential for automation of white blood cell isolation, magnetic bead DNA purification and isolation, and automated qPCR set-up platforms.

Actiphage® Rapid Blood Test
for HUMAN USE
Instructions for use

DOWNLOAD HERE
PBD process 2023

Watch the Actiphage assay workflow in the video.

developers of phage-based diagnostics

Contact Us
BIVDA member 2023

Registered Company Address
Grosvenor St Paul’s
Grosvenor House
11 St Paul’s Square
Birmingham
B3 1RB
United Kingdom

T: 01359 231856
info@pbdbio.com

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