Phage Based Diagnostics

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  • Home
  • About
    • About PBD Biotech
    • Industry Awards
    • Management Team
    • International
  • Actiphage
    • About Actiphage
    • Benefits of Actiphage
    • Rapid detection of mycobacterial disease
  • Bovine
    • Bovine TB
    • Johne’s Disease
    • Dairy Industry
  • Latest
    • Articles
    • Events
  • Case Studies
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Research

Actiphage® is phage-based technology that is able to detect extremely low levels of live mycobacteria in a wide range of species and samples.

The phage assay referred to in the following research papers is PBD Biotech’s patented Actiphage product.

Human TB

Proof of concept study uses high sensitivity Actiphage assay to identify low level Mycobacterium tuberculosis in immunocompetent patients with active and incipient TB:

Verma, R. et al, ‘A novel high sensitivity bacteriophage-based assay identifies low level M. tuberculosis bacteraemia in immunocompetent patients with active and incipient TB’, Clinical Infectious Diseases, ciz548

Jee Whang Kim et al, A novel bacteriophage-based assay stratifies tuberculosis risk in recent household contacts of pulmonary tuberculosis: A prospective observational cohort study

Presented at 32nd European Congress of Clinical Microbiology & Infectious Diseases April 2022

Further trials are underway in the UK, Canada and internationally. Please contact us for more information.

Johne’s Disease – cattle blood samples

The development and use of Actiphage® to detect a range of mycobacteria, including bovine TB and Johne’s disease, from blood samples [as mentioned above]:

B. M. C. Swift et al, ‘The development and use of Actiphage® to detect viable mycobacteria from bovine tuberculosis and Johne’s disease‐infected animals‘, Microbial Biotechnology (2019)

Detection of Johne’s disease infection of new-born calves in the first days of life:

Pelletier C. et al, ‘Detection of active infection of new-born calves by Mycobacterium avium subsp. paratuberculosis (MAP) in first days of life‘, Abstract presented at the European Association of Veterinary Laboratory Diagnosticians Congress (2018)

Improving rapid phage-based detection of Johne’s disease in the blood of experimentally infected cattle:

B. Swift et al, ‘Evaluation of the limitations and methods to improve rapid phage-based detection of viable Mycobacterium avium subsp. paratuberculosis in the blood of experimentally infected cattle‘, BMC Veterinary Research (2016), 12 (1), 115

Development and evaluation of a rapid phage-based method for detection of Johne’s disease in cattle blood:

Swift B. et al, ‘Development of a rapid phage-based method for detection of viable Mycobacterium avium subsp. paratuberculosis in blood in 48h‘, Journal of Microbiological Methods (2013), 94 (3), 175-179

Johne’s Disease – blood samples of other livestock

Development of a rapid method for detection of Johne’s disease in blood samples from farmed deer: 

Kubala A, et al. ‘Development of a method to detect Mycobacterium paratuberculosis in the blood of farmed deer using Actiphage® Rapid‘, Frontiers in Veterinary Science (2021) 8:665697 

MAP – Mycobacteria in dairy products

Survival of MAP in retail-bought pasteurised milk:

Gerrard Z.E. et al, ‘Survival of Mycobacterium avium subspecies paratuberculosis in retail pasteurised milk‘, Food Microbiology (2018), 74, 57-63

Detection of live MAP in powdered infant formula by phage assay:

Botsaris G. et al, ‘Detection of viable Mycobacterium avium subspecies paratuberculosis in powdered infant formula by phage-PCR and confirmed by culture‘, International Journal of Food Microbiology (2016), 216, 91-94

Bovine TB – cattle blood samples

The development and use of Actiphage® to detect a range of mycobacteria, including bovine TB and Johne’s disease, from blood samples:

B. M. C. Swift et al, ‘The development and use of Actiphage® to detect viable mycobacteria from bovine tuberculosis and Johne’s disease‐infected animals‘, Microbial Biotechnology (2019)

Read more about this paper >

Gatcombe Farm – a novel enhanced cattle testing programme, overseen by vet Dick Sibley to achieve Officially TB Free status: 

R. J. Sibley BVSc HonFRCVS, ‘The investigation of a persistent outbreak of bovine tuberculosis using a novel enhanced cattle testing programme and evaluation of environmental contamination‘, Paper presented at the British Veterinary Cattle Association Congress (2018)

Evidence of bovine tuberculosis in skin test positive cattle detected using 48 hour phage amplication testing protocol:

Convery T., Rees C. et al, ‘Evidence of Mycobacterium tuberculosis complex bacteraemia in intradermal skin test positive cattle detected using phage-RPA‘, Virulence (2016), 7 (7), 779-788

Multiplicity of infection:

Huynh A., Hill P., Swift B., Rees C, ‘Use of 2, 3, 5-Triphenyltetrazolium chloride (TTC) to assay the kinetics of phage and host interactions‘, Poster presented at Phages 2021

Developments in phage-based diagnostics

The Application of Bacteriophage Diagnostics for Bacterial Pathogens in the Agricultural Supply Chain: From Farm-to-Fork
Helen J. Jones, Christopher G. Shield, and Benjamin M.C. Swift
Published Online:16 Dec 2020 https://doi.org/10.1089/phage.2020.0042

Research articles

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Actiphage use as a DIVA test

Actiphage optimised to detect MAP in humans and to support use as ‘DIVA’

June 1, 2022
This is the first time Actiphage has been used to detect MAP in a human blood sample and also to identify a live infection in a vaccinated goat.
Actiphage use as a DIVA test

Actiphage optimised to detect MAP in humans and to support use as ‘DIVA’

June 1, 2022
This is the first time Actiphage has been used to detect MAP in a human blood sample and also to...
ECCMID

Actiphage TB diagnostic finds live mycobacterium tuberculosis in the blood of people with incipient TB

April 23, 2022
A clinical trial of Actiphage, a promising new diagnostic for tuberculosis infection, has shown for the first time that live bacteria can be detected in the blood of people with incipient TB infection.
ECCMID

Actiphage TB diagnostic finds live mycobacterium tuberculosis in the blood of people with incipient TB

April 23, 2022
A clinical trial of Actiphage, a promising new diagnostic for tuberculosis infection, has shown for the first time that live...
mycobacteria

Bacteriophage shows promise for detection and treatment of tuberculosis

March 7, 2022
Bacteriophages, viruses that infect and kill bacteria, offer the potential of a powerful tool for detecting and treating mycobacterial infections such as tuberculosis (TB), authors of a review in the journal Microorganisms* have reported.
mycobacteria

Bacteriophage shows promise for detection and treatment of tuberculosis

March 7, 2022
Bacteriophages, viruses that infect and kill bacteria, offer the potential of a powerful tool for detecting and treating mycobacterial infections...
Tomas Richardson

Disruptive phage technology attracts top scientist to PBD Biotech

January 18, 2022
“PBD Biotech has developed an exciting and potentially disruptive technology that uses bacteriophage to improve the molecular diagnosis of tuberculosis” says Dr Tomas Richardson.
Tomas Richardson

Disruptive phage technology attracts top scientist to PBD Biotech

January 18, 2022
“PBD Biotech has developed an exciting and potentially disruptive technology that uses bacteriophage to improve the molecular diagnosis of tuberculosis”...
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developers of phage-based diagnostics

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