Phage Based Diagnostics

01359 231856

Twitter Youtube Linkedin Envelope
  • Home
  • About
    • About PBD Biotech
    • Industry Awards
    • Management Team
    • International
  • Actiphage
    • About Actiphage
    • Benefits of Actiphage
    • Rapid detection of mycobacterial disease
  • Bovine
    • Bovine TB
    • Johne’s Disease
  • Latest
    • Articles
    • Events
  • Case Studies
  • Research
  • Contact
    • Product Order Form
    • Contacting us
Menu
  • Home
  • About
    • About PBD Biotech
    • Industry Awards
    • Management Team
    • International
  • Actiphage
    • About Actiphage
    • Benefits of Actiphage
    • Rapid detection of mycobacterial disease
  • Bovine
    • Bovine TB
    • Johne’s Disease
  • Latest
    • Articles
    • Events
  • Case Studies
  • Research
  • Contact
    • Product Order Form
    • Contacting us
Search
Generic filters

Rapid detection of mycobacterial disease

Actiphage is unique as it is able to detect and identify live mycobacteria at a very early stage of infection, unlike other tests which rely on an immune response

Loading...

About Actiphage

Actiphage is unique as it is able to detect and identify live mycobacteria at a very early stage of infection,...

Benefits of Actiphage

Actiphage offers benefits over tests that rely on an immune response.

A major problem with many of the current diagnostics is they use the host immune response to diagnose infection. A key feature of mycobacteria is their ability to avoid the host’s immune system, resulting in inconsistent detection of infected individuals.

The alternative, gold-standard, diagnostic is culture, but this is also difficult as M. tuberculosis can take up to 12 weeks to culture.

So, a rapid, sensitive, alternative approach is required to understand, control and eradicate TB.

Actiphage uses a bacteriophage (literally: ‘bacteria eater’), a virus that is specific to a particular bacterium. The phage destroys the host cell, releasing its DNA, which can then be used as a target for detection and identification within hours.

Simple yes/no answer

The Actiphage assay combines phage technology (to detect only living bacteria) with traditional qPCR (which is extremely sensitive and specific for target DNA sequences) to create a new, highly sensitive and specific diagnostic assay that detects only live, disease-causing bacteria. 

It provides a simple yes/no answer – removing the confusion caused by current testing methods. 

Identifies a range of mycobacterial disease

Actiphage uses PCR primers to identify signature sequences known to be present in the genome of the bacteria. These sequences differ between the strains of the mycobacteria so the Actiphage test can be used to provide a number of mycobacterial tests.

Mycobacterial diseases detected

Actiphage also offers many benefits for diagnosis of human tuberculosis (M. tuberculosis).

In cattle, Actiphage can detect Mycobacterium bovis (M. bovis), which causes Bovine Tuberculosis (bTB) and M. avium subsp paratuberculosis (MAP), which causes Johne’s disease.

In addition, it can be used to identify both bTB and Johne’s disease in a range of different species, including sheep, deer and exotic captive species and also avian infections caused by M. avium.

 

 

Related content

Loading...
ECCMID

Actiphage® TB diagnostic finds live mycobacterium tuberculosis in the blood of people with incipient TB

April 23, 2022
A clinical trial of Actiphage, a promising new diagnostic for tuberculosis infection, has shown for the first time that live bacteria can be detected in the blood of people with incipient TB infection.
ECCMID

Actiphage® TB diagnostic finds live mycobacterium tuberculosis in the blood of people with incipient TB

April 23, 2022
A clinical trial of Actiphage, a promising new diagnostic for tuberculosis infection, has shown for the first time that live...
mycobacteria

Bacteriophage shows promise for detection and treatment of tuberculosis

March 7, 2022
Bacteriophages, viruses that infect and kill bacteria, offer the potential of a powerful tool for detecting and treating mycobacterial infections such as tuberculosis (TB), authors of a review in the journal Microorganisms* have reported.
mycobacteria

Bacteriophage shows promise for detection and treatment of tuberculosis

March 7, 2022
Bacteriophages, viruses that infect and kill bacteria, offer the potential of a powerful tool for detecting and treating mycobacterial infections...
Tomas Richardson

Disruptive phage technology attracts top scientist to PBD Biotech

January 18, 2022
“PBD Biotech has developed an exciting and potentially disruptive technology that uses bacteriophage to improve the molecular diagnosis of tuberculosis” says Dr Tomas Richardson.
Tomas Richardson

Disruptive phage technology attracts top scientist to PBD Biotech

January 18, 2022
“PBD Biotech has developed an exciting and potentially disruptive technology that uses bacteriophage to improve the molecular diagnosis of tuberculosis”...
huynh-phg21-poster-final-web

Multiplicity of infection – Albert Huynh presents research at Phages 2021

September 22, 2021
Albert’s research, ‘Use of 2, 3, 5-Triphenyltetrazolium chloride (TTC) to assay the kinetics of phage and host interactions’, is sponsored by PBD Biotech through the BBSRC CASE initiative, and was co-authored by fellow Nottingham student Philip J. Hill and PBD...
huynh-phg21-poster-final-web

Multiplicity of infection – Albert Huynh presents research at Phages 2021

September 22, 2021
Albert’s research, ‘Use of 2, 3, 5-Triphenyltetrazolium chloride (TTC) to assay the kinetics of phage and host interactions’, is sponsored...
1 2 3 Next »

developers of phage-based diagnostics

Contact Us

PBD Biotech Ltd
Link House, Elm Farm Park,
Great Green, Thurston,
Suffolk IP31 3SH,
United Kingdom

Registered Company Address
Ingenuity Centre,
University of Nottingham,
Innovation Park, Triumph Rd,
Nottingham NG7 2TU,
United Kingdom

T: 01359 231856
info@pbdbio.com

Privacy and cookies policy

© 2022 PBD Biotech Ltd

design: out of house

  • Follow Us!
PBD Biotech

Health Protection Scotland has launched a consultation on bovine tuberculosis diagnostic testing - but only the skin test? Why not the use of a blood test, pre-movement to keep Scotland OTF status by detecting early infection? @P_H_S_Official @BritishVets

Read More
PBD Biotech

Johne's Disease is a ticking time bomb in the dairy herd, now Actiphage provides a pre-breeding blood test to stop its transmission. We explain the relevance of new research that proves the link between mycobacteria in the blood and the development of TB

Read More
PBD Biotech

Delighted to be taking our Actiphage tuberculosis blood test to Zambia, in a collaborative project with @ZambartResearch, @LSHTM, @LeicResearch The aim is to test its sensitivity in a real world setting. #Tuberculosis #EndTB #InvestToEndTB

Read More